Coding

Part:BBa_K2201030:Design

Designed by: Lennard Karsten   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


cas9 from Streptococcus pyogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cas9 from Streptococcus pyogenes


Source

cas9 from Streptococcus pyogenes

References